IPTG(367-93-1)

Overview: Isopropyl thiogalactoside (IPTG) is a powerful inducer, which is not metabolized by bacteria and is very stable, so it is widely used in the laboratory. IPTG is often used to induce β- Cloning experiment of galactosidase activity. It is often used in combination with X-Gal or Bluo-Gal for blue-white screening of recombinant bacterial colonies, which can induce the expression of lac operon in Escherichia coli. IPTG acts by binding with lacI repressor protein and changing its conformation to prevent β- Inhibition of the gene lacZ encoding galactosidase.

Purpose: Commonly used molecular biological reagents, commonly used in blue and white spot screening and IPTG-induced protein expression in bacteria

The purpose is β- Galactosidase and β- Inducer of galactose permase, not β- Galactoside hydrolysis is the substrate of thiogalactosyltransferase

Purpose: IPTG is often used for induction β- Cloning experiment of galactosidase activity. It is often used in combination with X-Gal or Bluo-Gal for blue-white screening of recombinant bacterial colonies, which can induce the expression of lac operon in Escherichia coli. IPTG acts by binding with lacI repressor protein and changing its conformation to prevent β- Inhibition of the gene lacZ encoding galactosidase.

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